EXPERIMENT2

EXPERIMENT2 24.08.2019
 EXPERIMENT2 Essay

UNIVERSITI TEKNOLOGI MARA

BIO 411

CELL BIOLOGY

PRACTICE a few

REDOX -REACTION/CATALASE ACTIVITY

GROUP MEMBERS: Syazani Zahier Rubbish bin Zakaria (2014292204)

Nur Fatin Syamimi Binti Ahmad Tarmize (2014236664)

Zuliakha Binti Zolkafali(2014279698)

Nur Izzati Binti Azizan(2014284578)

GROUP: ASB1Cb

DATE OF SUBMISSION: doze November 2014

INSTRUCTOR: Miss Ellia Kartini Mujar

GOALS

1 . To demonstrate reduction-oxidation response on living tissue. installment payments on your To review enzyme catalase on plant and animal tissues.

a few. To measure the rate on enzyme reaction.

some. To assess enzyme effect.

INTRODUCTION

With this lab activity, we explore on the enzyme that is present in the skin cells of many living tissues. The enzyme can be catalase. Catalase functions in speeding up reaction in our skin cells that break up hydrogen peroxide, a poisonous substance right into a harmless compound which is drinking water and oxygen. Hydrogen peroxide (H2O2) may be the by-product of several normal cell respirations mainly in our body system for it to function properly. Nevertheless , H2O2 can be described as toxic compound that can be damaging for your body once is actually not processed properly. If the cell are not able to break down H2O2 into a undamaging substance such as water and hydrogen, we all as the multicellular affected person will be poisoned to loss of life. For experiment 3 the experiment is designed to measure the price of response by testing the amount of base remaining following some length of reaction time. H2O2 is the substrate that will convert to H2O2+ O2 with time. Hence, this will likely give us roundabout measure of the substrate converted to product plus the rate of reaction. The remaining substrate in the reaction is equal to your initial amount of the substrate minus the amount transformed into product by enzymatic actions. MATERIALS

3% hydrogen peroxide

Sulphuric chemical p (1M)

Potassium permanganate option

Soaked beans, potatoes, apple and poultry meat

Razor blade blades

Lime juice

Test pipes and shelves

Ice, beakers

Watch plates

Burettes

Water bath

Cone-shaped flask

STRATEGIES

Experiment you: Observing redox reaction

1 . Three little pieces of spud and apple were cut and positioned pair simply by pair in to the 3 observe plates. 2 . The 1st watch dish was still left exposed to air flow, the second one was added with normal water while the third one was added together with the lime juice. three or more. After forty-five minutes, the declaration was compared and recorded for all the slices in the a few watch a glass. Experiment a couple of: Comparing catalase activity in living cells 1 . 2mL of hydrogen peroxide solution was placed into four clean test pipes. 2 . Slices of spud, apple, chicken breast meat and 3 components of beans were prepared. several. Each of the evaluation tubes had been then added with the ready slices and the 3 coffee beans respectively to get test pontoons 1, a couple of, 3 and 4. four. The reaction that develops once the substances react with all the hydrogen peroxide was discovered and graded based on the amount of bubbles created, from the size 0-5 with 0 as no effect while a few as very quick reaction. five. The temperatures of the check tubes was also experienced using hands and was recorded. Experiment several: Effect of Temperature on Catalase Activity

1 . 4 test tubes had been added with 3mL of hydrogen peroxide. The test tubes was placed into different beaker to acquire different temperature of the hydrogen peroxide answer. 2 . Test out tube 1-4°C, Test tube 2-24°C, Test out tube 3-34°C, Test tube 4-44°C respectively. 3. The test tubes were left to get 20 mins to obtain the temps. 4. Following 20 minutes, each evaluation tube was added with 6 components of beans. The response was kept to occur for about 1 tiny. 5. Then simply, 3. five mL of sulphuric chemical p was added into every test pipe to stop the reaction immediately. 6. The content in the test pipes were poured into conical flask and one of the evaluation tubes content material was titrated with KMnO4 until the blend turns lilac. 7. The amount of KMnO4 employed was noticed and recorded.

RESULTS

TEST OUT TUBE

1

2

three or more

NO . OF SLICED ITEM

2

two

2

CHANGE OF COLOUR

Brown

Dark brown

Brown

TIMING

45 minutes

forty-five minutes

45 minutes...

Referrals: http://www.xula.edu/chemistry/documents/biolab/Carroll%20lab%20Chap%208.pdf ( accessed about 12 Nov 2014)

Eyster H. C. (1950). Effect of The Temp On Catalase Activity. The Ohio Record of Scientific research, v50 n6, 273-277.

Morgulis H., Beber M., Rabkin I actually. (1926). Studies On The A result of The Heat On The Catalase Reaction Log of Biology Chemistry, sixty-eight: 521-533.

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